Synopsis Genetic Engineering is based on an understanding of the molecular aspects of genetic processes occurring in nature. The techniques of gene manipulation enable us to transcend conventional biological boundaries to create completely novel gene combinations and even new genes. This book presents the principles of gene manipulation and its associated techniques in sufficient details and also covers Biological replication of DNA, Protein synthesis and its regulation, Nucleic acid, Ribonucleic acid, Restriction endonuclease, Tools and Manipulation, Regulation, Application and Vaccines etc. Besides useful as a textbook to undergraduate and postgraduate students, it is useful to many experienced researchers as an informative references source.