The present publication has been prepared to provide appropriate knowledge of the sound strategies and essential techniques with their application to biotechnological enterprises. This text aims to provide the essential knowledge of the core processes, thereby enabling readers to understand and contribute to the practical application of these techniques. The publication covers the microscope, Centrifuges, Spectrophotometry, PH, Osmometry, Auto Analyzer, Chromatography, Gas Chromatography, Electrophoresis and Flame Photometry etc. The publication is useful not only to the undergraduate and postgraduate students but also to the academic professionals as an informative reference source.