Contents: 1. Fish, fisheries and biotechnology. 2. Animal cell and tissue culture. 3. Fish genetics and development of transgenic fishes. 4. Enzymes in genetic engineering (nucleic acid enzymology). 5. Fish genomics: trends and prospects. 6. Gene cloning vectors. 7. Techniques for genetic engineering. 8. Gene cloning. 9. Cryopreservation in fishes. 10. Techniques and genetic requirements for transgenic fish production. 11. Applications of transgenic fishes and biotechnology. 12. Biotechnology in health management for aquaculture. Glossary. Selected bibliography.